1. What is Enzyme Inhibitor Dissociation Constant?

Definition: This calculator determines the dissociation constant of an enzyme-inhibitor complex based on the inhibitor concentration and enzyme modifying factor.

Purpose: It helps biochemists and researchers quantify the strength of enzyme-inhibitor interactions in biochemical systems.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( K_{ei} \) — Enzyme inhibitor dissociation constant (mol/m³)
• \( I \) — Inhibitor concentration (mol/m³)
• \( α \) — Enzyme modifying factor (dimensionless)

Explanation: The dissociation constant is calculated by dividing the inhibitor concentration by the enzyme modifying factor minus one.

3. Importance of Dissociation Constant

Details: The dissociation constant provides insight into inhibitor potency - lower values indicate stronger binding between enzyme and inhibitor.

4. Using the Calculator

Tips: Enter the inhibitor concentration in mol/m³ and the enzyme modifying factor (must be > 1). The modifying factor typically has ±5% variability.

5. Frequently Asked Questions (FAQ)

Q1: What does the enzyme modifying factor represent?
A: It describes how much the inhibitor alters the enzyme's kinetic parameters, with higher values indicating stronger effects.

Q2: Why must the modifying factor be greater than 1?
A: A factor of 1 would mean no inhibition, and values <1 would indicate activation rather than inhibition.

Q3: What are typical values for inhibitor concentrations?
A: In biochemical systems, inhibitor concentrations typically range from micromolar (10⁻⁶ mol/m³) to millimolar (10⁻³ mol/m³).

Q4: How accurate is this calculation?
A: The ±5% variability in the modifying factor should be considered when interpreting results.

Q5: What are common applications of this calculation?
A: Used in drug development, enzyme kinetics studies, and biochemical pathway analysis.

Dissociation Constant of Enzyme given Modifying Factor of Enzyme Calculator© - All Rights Reserved 2025