Dissociation Constant Of Enzyme Substrate Complex Given Modifying Factor Of Enzyme Substrate Calculator

Formula Used:

\[ K_i' = \frac{I}{\alpha' - 1} \]

Enzyme Substrate Dissociation Constant (K_i'):

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1. What is the Enzyme Substrate Dissociation Constant?

The Enzyme Substrate Dissociation Constant (K_i') represents the equilibrium constant for the dissociation of an enzyme-substrate complex in the presence of an inhibitor. It quantifies the affinity between the enzyme and substrate when modified by an inhibitor.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ K_i' = \frac{I}{\alpha' - 1} \]

Where:

\( K_i' \) — Enzyme Substrate Dissociation Constant (mol/m³)
\( I \) — Inhibitor Concentration (mol/m³)
\( \alpha' \) — Enzyme Substrate Modifying Factor

Explanation: This formula calculates the modified dissociation constant when an inhibitor affects the enzyme-substrate interaction, where α' represents the factor by which the inhibitor modifies the substrate binding.

3. Importance of Dissociation Constant Calculation

Details: Accurate determination of the dissociation constant is crucial for understanding enzyme kinetics, inhibitor effects, and designing effective enzyme inhibition studies in biochemical research and drug development.

4. Using the Calculator

Tips: Enter inhibitor concentration in mol/m³ and the enzyme substrate modifying factor. The modifying factor must be greater than 0 and not equal to 1 for valid calculation.

5. Frequently Asked Questions (FAQ)

Q1: What does the Enzyme Substrate Modifying Factor represent?
A: The modifying factor (α') quantifies how much an inhibitor affects the binding affinity between the enzyme and substrate.

Q2: Why can't the modifying factor be equal to 1?
A: When α' = 1, the denominator becomes zero, making the equation undefined as the inhibitor would have no effect on the dissociation constant.

Q3: What are typical values for the dissociation constant?
A: Values vary widely depending on the specific enzyme-substrate-inhibitor system, ranging from nanomolar to millimolar concentrations.

Q4: How is this different from the standard dissociation constant?
A: K_i' represents the modified dissociation constant in the presence of an inhibitor, while K_d typically refers to the dissociation constant without inhibitors.

Q5: What units should be used for concentration values?
A: While mol/m³ is used here, consistent units must be maintained throughout the calculation. Conversions may be needed for different unit systems.