Beer-Lambert Law:
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The Beer-Lambert Law describes the relationship between the absorption of light and the properties of the material through which the light is traveling. It states that the absorbance of a solution is directly proportional to the concentration of the absorbing species and the path length.
The calculator uses the Beer-Lambert Law:
Where:
Explanation: The equation calculates the original intensity of radiation before it passes through a solution, based on how much radiation is transmitted through the solution.
Details: Calculating incident radiation intensity is crucial in spectroscopy, chemical analysis, and environmental monitoring to understand light absorption properties of materials and determine concentrations of solutions.
Tips: Enter all values in appropriate units. Ensure all inputs are positive numbers. The calculator will compute the intensity of incident radiation based on the transmitted intensity and solution properties.
Q1: What is the difference between incident and transmitted radiation?
A: Incident radiation is the light that strikes a surface, while transmitted radiation is the light that passes through a material.
Q2: What factors affect the molar extinction coefficient?
A: The molar extinction coefficient depends on the substance, solvent, temperature, and wavelength of light used.
Q3: When is the Beer-Lambert Law not applicable?
A: The law may not hold at very high concentrations, in scattering media, or when there are chemical associations between molecules.
Q4: What are typical units for concentration in spectroscopy?
A: While mol/m³ is used here, mol/L (molarity) is also commonly used in practice.
Q5: How does cell thickness affect the measurement?
A: Thicker cells provide longer path lengths, which can increase sensitivity for dilute solutions but may cause deviations from the Beer-Lambert Law at high concentrations.